Measurement of human dopamine-beta-hydroxylase in serum by homologous radioimmunoassay.
نویسندگان
چکیده
Human serum dopamine-beta-hydroxylase [3,4-dihydroxyphenylethylamine, ascorbate: oxygen oxidoreducatse (beta-hydroxylating), EC 1.14.17.1] levels have been determined in 52 samples with a newly devised radioimmunoassay that utilizes purified human enzyme and its specific antiserum. The values obtained were in excellent agreement (correlation coefficient r equals 0.98) with those obtained by measuring enzymatic activity in the same sera. Either assay technique, enzymatic or homologous radioimmunoassay, can be used for measurement of the enzyme in normal human serum.
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ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 72 2 شماره
صفحات -
تاریخ انتشار 1975